beas 2b normal human bronchial epithelial cell line  (ATCC)

Journal: Discover Oncology

Article Title: Endoplasmic reticulum stress-related genes play a role in the prognosis of lung adenocarcinoma

doi: 10.1007/s12672-025-04240-1

Figure Lengend Snippet: Quantitative PCR showing the expression of seven ER stress-related genes in normal human bronchial epithelial cell line BEAS-2B and LUAD cell lines (A549, NCI-H1975, and HCC1833). *, **, and *** represent p < 0.05, p < 0.01, and p < 0.001

Article Snippet: Normal human bronchial epithelial cell line BEAS-2B and LUAD cell lines (A549, NCI-H1975, and HCC1833) were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: Translational Lung Cancer Research

Article Title: A public data-based molecular classification of small cell lung cancer by neuroactive signaling networks unveils distinct microenvironment landscapes and immunotherapy-related prognostic biomarkers

doi: 10.21037/tlcr-2025-620

Figure Lengend Snippet: Identification of prognostic genes in NRS and Validation. (A) Forest plot shows prognosis-associated genes within NRS. (B) In George’s cohort, the distribution of high and low expression of NPPC. Cutting off value was determined using the survminer R package. High expression level of NPPC is associated with poor patient prognosis. (C,D) t-SNE visualization of cells shows the expression of NPPC and its receptor NPR2 in single-cell RNA-seq data. (E) Representative areas of HE and IHC of NPPC expression in tumorous tissues from three SCLC patients. Scale bar, 50 µm. (F-G) qPCR validation of NPPC and NPR2 expression across various human normal epithelial and SCLC cell lines. (H) Transwell migration assay of SCLC-1 cells treated with CNP-38 (product of the NPPC gene) or DMSO (control) for 12 hours (0.5% crystal violet dye, ×100). (I) Data processing and analysis of transwell migration assay. ***, P<0.001; CI, confidence interval; CNP, C-type natriuretic peptide; DMSO, dimethyl sulfoxide; HE, hematoxylin and eosin staining; IHC, immunohistochemistry; NPPC, natriuretic peptide C; NPR2, natriuretic peptide receptor B; NRS, nomogram/prognostic risk score; qPCR, quantitative polymerase chain reaction; RNA-seq, ribonucleic acid sequencing; SCLC, small cell lung cancer; t-SNE, t-distributed stochastic neighbor embedding.

Article Snippet: Human SCLC cell lines (H446, H146, H1688, H526), human normal bronchial epithelial cell lines (16HBE, BEAS-2B), human lung adenocarcinoma cell line (A549), and human large cell lung cancer cell line (H460) were purchased from the American Type Culture Collection (ATCC, RRID: CVCL_1562 for H446, CVCL_1473 for H146, CVCL_1487 for H1688, CVCL_1569 for H526, CVCL_0021 for 16HBE, CVCL_0168 for BEAS-2B, CVCL_0023 for A549, and CVCL_0459 for H460).

Techniques: Biomarker Discovery, Expressing, RNA Sequencing, Transwell Migration Assay, Control, Staining, Immunohistochemistry, Real-time Polymerase Chain Reaction, Sequencing

Journal: Translational Oncology

Article Title: Single-cell RNA sequencing reveals palmitoylation-driven cellular heterogeneity and prognostic biomarkers in lung adenocarcinoma

doi: 10.1016/j.tranon.2025.102501

Figure Lengend Snippet: Validation of prognostic gene expression in LUAD cell lines. (A) qRT-PCR analysis of aspartate beta-hydroxylase (ASPH), CKS2, and IRX3 expression in BEAS-2B (normal bronchial epithelial cells), A549, and PC-9 (LUAD cell lines). Data are presented as mean ± SD, P < 0.05.

Article Snippet: Normal human bronchial epithelial cells (BEAS-2B) were obtained from ATCC and grown in DMEM.

Techniques: Biomarker Discovery, Gene Expression, Quantitative RT-PCR, Expressing

Journal: Translational Oncology

Article Title: Single-cell RNA sequencing reveals palmitoylation-driven cellular heterogeneity and prognostic biomarkers in lung adenocarcinoma

doi: 10.1016/j.tranon.2025.102501

Figure Lengend Snippet: ASPH is overexpressed in lung adenocarcinoma tissues and cell lines, and its silencing impairs tumour‐cell proliferation. (A) qRT-PCR analysis of ASPH mRNA levels in paired adjacent normal (“Adjacent”) and tumour (“Cancer”) lung specimens. ** P < 0.01 versus adjacent. (B) Basal ASPH expression measured by qRT-PCR in the normal bronchial epithelial cell line BEAS-2B and five LUAD cell lines (NCI-H1975, HCC827, A549, Calu-3, NCI-H2228). Data are mean ± SD; ns, not significant; ** P < 0.01; *** P < 0.001; **** P < 0.0001 versus BEAS-2B (C) Confirmation of ASPH knockdown in HCC827 and NCI-H2228 cells transfected with si-ASPH or non-targeting control (si-NC). Relative ASPH mRNA levels were quantified by qRT-PCR. Data are mean ± SD; ** P < 0.01; *** P < 0.001 versus si-NC. (D, E) CCK-8 assays showing growth curves of HCC827 (D) and NCI-H2228 (E) cells over 4 days post-transfection. OD₄₅₀ readings are plotted as mean ± SD from three independent experiments; ** P < 0.01; *** P < 0.001 versus si-NC at day 4.

Article Snippet: Normal human bronchial epithelial cells (BEAS-2B) were obtained from ATCC and grown in DMEM.

Techniques: Quantitative RT-PCR, Expressing, Knockdown, Transfection, Control, CCK-8 Assay

Journal: The Journal of Biological Chemistry

Article Title: Nucleolar protein DCAF13 promotes non–small cell lung cancer cell proliferation via facilitating rDNA transcription and ribosome biogenesis

doi: 10.1016/j.jbc.2025.110656

Figure Lengend Snippet: DCAF13 promotes non–small cell lung cancer progression. A , DCAF13 mRNA expression level by UALCAN. B , association between DCAF13 mRNA expression and prognosis in TCGA LUAD samples by Kaplan–Meier analysis (log-rank test). C , association between DCAF13 mRNA expression and pathologic staging. D , western blot results showing the DCAF13 expression level in NSCLC adjacent paraneoplastic tissues (NT) and tumor tissues (T). E , expression levels of DCAF13 in lung cancer cell lines (A549, NCI-H358, CALU-1, and H1299) and normal pulmonary bronchial epithelial cell (BEAS-2B) were evaluated by western blot. F , deletion of DCAF13 was confirmed at the protein level in different clones of H1299 and A549 cells by immunoblotting. G , growth curve assay for D CAF13 deletion H1299 and A549 cells. Cells (100,000) were plated in six-well culture dishes and cells were counted on days 1, 2, and 3. The data (N = 3 per cell line) are plotted as mean ± SEM. ∗∗∗ p < 0.001, unpaired t test. H , DCAF13 deletion inhibits colony formation in H1299 and A549 cells. Data are presented as mean ± SEM for N = 3 per cell line. ∗∗∗ p < 0.001. I , wound healing assay was performed for evaluating the migration ability of DCAF13 deletion H1299 and A549 cells. J , the expression of several key cell metastatic signal regulators, vimentin, MMP-9, E-cadherin, slug, snail, N-cadherin, and CCT8 were examined by qPCR. ∗∗∗ p < 0.001, unpaired t test. K , Transwell migration assay with the 24-well transwell system and quantitative analysis (original magnification: × 100). L , the numbers of migrated cells were counted in five representative high-power fields per transwell plate, ∗∗ p < 0.01. DCAF13, DDB1- and CUL4-associated factor 13; LUAD, lung adenocarcinoma; NSCLC, non–small cell lung cancer; qPCR, quantitative real-time PCR.

Article Snippet: Human NSCLC cell lines (A549, H1299, Calu-1, and NCI-H358), normal pulmonary bronchial epithelial cells (BEAS-2B), and human embryonic kidney cells (293T) were purchased from the American Type Culture Collection.

Techniques: Expressing, Western Blot, Clone Assay, Wound Healing Assay, Migration, Transwell Migration Assay, Real-time Polymerase Chain Reaction